Huff Creek is a tributary of the Guyandotte River, 21.2 miles (34.1 km) long, in southern West Virginia in the United States. Via the Guyandotte and Ohio rivers, it is part of the watershed of the Mississippi River, draining an area of 52 square miles (130 km2) in a rural area on the unglaciated portion of the Allegheny Plateau. The creek was named after Peter Huff, an early settler.